Fig. 1

Effect of Cx26I30N and D50Y mutations on protein expression and localization. Merged images of Cx26WT, I30N and D50Y transfected HeLa cells that were co-stained with phalloidin for actin (green) and Cx26 antibody (red). Blue is for DAPI staining of the nucleus. White arrow head shows the gap junction plaques formed between adjacent Cx26WT expressing cells. Red arrow heads point the cell-to-cell contact sites between neighboring cells of Cx26I30N and D50Y. Cells with only green and blue signals indicate untransfected cells. Scale bar 10 μm