Fig. 4
From: IL-27 inhibits the TGF-β1-induced epithelial-mesenchymal transition in alveolar epithelial cells
IL-27 affects TGF-β1-mediated EMT in AECs via the TGF-β1/Smad signalling pathway. a The protein levels of TGF-βR1, pSmad1, pSmad3, pSmad5 and their total protein were measured by western blot after treatment with IL-27/and TGF-β1 for 1 h. b, c A549 cells were treated with 40 ng/ml of TGF-β1 and 100 ng/ml of IL-27, a Smad3 specific inhibitor SIS3, or TGF-β1 and IL-27 and/or SIS3 for 48 h. Real-time PCR and western blotting was performed to analyse the expression of E-cadherin, β-catenin, vimentin and α-SMA. The results were quantified using Image J software. All data are shown as the mean ± SD (n = 3). Statistical significance was assessed by one-way ANOVA and Tukey’s post hoc test using GraphPad Prism Version 5.0a software. *P < 0.05; **P < 0.01; ***P < 0.001