Fig. 3
From: Spatiotemporal patterns of sortilin and SorCS2 localization during organ development
Expression of Sortilin in the developing alimental canal and submandibular gland. Immunohistofluorescence was performed on sagittal sections (10 μm) of embryos on embryonic day E13.5, E15.5 and E17.5. a. Sortilin is expressed in the epithelium of the midgut at E13.5 (a, a’) but is not observed in the small intestine from E15.5 and onwards, apart from a small subset of epithelial cells (b-c’). b. Sortilin is highly expressed in the epithelium of the developing submandibular gland and is primarily apically located (d-e’). c. Sortilin is moderately expressed in the epithelium of the developing ventricle (f, f’) and the large intestine at E17.5 (g, g’). Green channel is sortilin, red channel is calretinin and blue channel is Hoechst nuclear stain. Scale bars = 50 μm. Ec, presumptive enteroendocrine cell; Li, large intestine; Lu, lumen; Mg, midgut; Pc, pancreas; Si, small intestine; Su, submandibular gland branches; Ve, ventricle; Vi, intestinal villus