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Fig. 3 | BMC Cell Biology

Fig. 3

From: Microvesicles derived from hypoxia/reoxygenation-treated human umbilical vein endothelial cells promote apoptosis and oxidative stress in H9c2 cardiomyocytes

Fig. 3

H/R-EMVs exhibited pro-apoptotic effect on H9c2 cells. a Hoechst 33258 staining was used to observe the apoptotic cells after treatment with 10, 30, 60 μg/mL H/R-EMVs for 6 h (100×). The arrows show apoptotic cells. The untreated cells serve as Control. b Annexin V-FITC/PI double staining. The results were interpreted in the following fashion: cells in the lower-left quadrant (Annexin V−/PI−) represent living cells; those in the lower-right quadrant (Annexin V+/PI−) represent early apoptotic cells; those in the upper-right quadrant (Annexin V+/PI+) represent late apoptotic cells; and those in the upper-left quadrant (Annexin V−/PI+) represent necrotic cells. c The apoptotic rate of H9c2 cells was quantified according to flow cytometry analysis. d Caspase 3 activity of H9c2 cells was measured after treatment with 10, 30, 60 μg/mL H/R-EMVs for 6 h. e Representative images of immunoblots with antibodies against Bcl-2, Bax, β-actin, respectively among groups. β-actin serves as a loading control. * p < 0.05, ** p < 0.01, *** p < 0.001 versus Control; # p < 0.05, ## p < 0.01, ### p < 0.001 versus H/R-EMVs 10 μg/mL; & p < 0.05, &&& p < 0.001 versus H/R-EMVs 30 μg/mL

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