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Fig. 4 | BMC Cell Biology

Fig. 4

From: Matrix metalloproteinase 9 induces endothelial-mesenchymal transition via Notch activation in human kidney glomerular endothelial cells

Fig. 4

rMMP-9 induces EndoMT in HKGECs. a rMMP-9-induced morphological changes in HKGECs were examined using phase contrast microscopy. b Indirect immunofluorescence staining of CD31, α-SMA, VE-cadherin, and vimentin was performed in HKGECs cultured in Endothelial Cell Medium in the presence or absence of rMMP-9 (2 μg/ml). c Western blot analysis of CD31, VE-cadherin and α-SMA in HKGECs treated or not with rMMP-9 (2 μg/ml). β-actin was used as a loading control. Original magnification × 200. Data are expressed as mean ± SEM with n ≥ 3 for each experimental group, *P < 0.05, **P < 0.01

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