Skip to main content
Fig. 5 | BMC Cell Biology

Fig. 5

From: Myc-binding protein orthologue interacts with AKAP240 in the central pair apparatus of the Chlamydomonas flagella

Fig. 5

The recognition of AKAP240 by recombinant FAP174 and RII in vitro. (a) The blots were probed with recombinant 6His-tagged RIIa D/D protein and revealed by HRP-conjugated anti-His antibody. The polypeptide migrated above 250 kDa in the axonemes of WT, pf14 and pf20 but were absent in mutants lacking the central pair, as expected of AKAP240. (b) 6His-FAP174 overlays also show the same band above 250 kDa. 6His-FAP174 also reacted with two other proteins. The polypeptide migrated above 250 kDa in the axonemes of WT, pf14 and pf20 but were absent in mutants lacking the central pair. (c) Control overlay blots with WT and mutant axonemes were probed with the anti-His antibody coupled to HRP without the interacting proteins

Back to article page

BMC Molecular and Cell Biology

ISSN: 2661-8850

Contact us