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Table 1 List of genes and proteins used/generated in the current study

From: Myc-binding protein orthologue interacts with AKAP240 in the central pair apparatus of the Chlamydomonas flagella

No. Insert Vector Protein product Comment Ref
1 fap174 pET28a 6His FAP174 c-Myc Binding protein ortholog Current study
2 RSP3 pGEX2T GST-RSP3 Radial spoke AKAP full length [17]
3 Δrsp3 (96–180) pGEX2T GST-RSP3-AH 96-180 Truncated RSP-3 with Amphipathic helix (96–180 a.a.) that binds to proteins with Dimerization and Docking domains [14]
4 arl6 pET28a ARL6 Full length GTP-binding protein From Jacinta D’Souza laboratory
5 rIIa D/D pET15b RIIa D/D Regulatory subunit of PKA, truncated protein (1–44 a.a.) with Dimerization and Docking domain Gift from Susan Taylor laboratory [39]