Table 1 List of genes and proteins used/generated in the current study
No. | Insert | Vector | Protein product | Comment | Ref |
|---|---|---|---|---|---|
1 | fap174 | pET28a | 6His FAP174 | c-Myc Binding protein ortholog | Current study |
2 | RSP3 | pGEX2T | GST-RSP3 | Radial spoke AKAP full length | [17] |
3 | Δrsp3 (96–180) | pGEX2T | GST-RSP3-AH 96-180 | Truncated RSP-3 with Amphipathic helix (96–180 a.a.) that binds to proteins with Dimerization and Docking domains | [14] |
4 | arl6 | pET28a | ARL6 | Full length GTP-binding protein | From Jacinta D’Souza laboratory |
5 | rIIa D/D | pET15b | RIIa D/D | Regulatory subunit of PKA, truncated protein (1–44 a.a.) with Dimerization and Docking domain | Gift from Susan Taylor laboratory [39] |