Fig. 1
From: Early changes in the metabolic profile of activated CD8+ T cells
Stimulation of CD8+ T cells with antibodies leads to rapid ATP consumption. Purified CD8+ T cells were treated with either soluble CD3/CD8 mAbs or OT-I tetramers (PMHC) for the indicated time periods. a Cellular ATP production was analyzed using the ATP-Assay Kit. n = 6 (b) AMPK activation was determined by Western blotting. Phospho-ERK staining was included to show effective stimulation. Total AMPK and actin are included as loading controls. c Quantification of AMPK phosphorylation within 60 min after stimulation, n = 3