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Fig. 7 | BMC Cell Biology

Fig. 7

From: Specific localization of nesprin-1-α2, the short isoform of nesprin-1 with a KASH domain, in developing, fetal and regenerating muscle, using a new monoclonal antibody

Fig. 7

Immunofluorescent staining for nesprin-1 in human skeletal muscle sections with regenerating muscle fibres. Individual microscopic fields are shown as paired images. The left image of each pair shows antibody stain (green) and the right image of each pair shows the merged antibody (green) and DAPI (blue) nuclear stain. a Serial sections from a patient with Duchenne muscular dystrophy, stained with mAb N1alpha2-1H2 (upper frame) and neonatal myosin (MHCn; regenerating fibres; lower frame). Staining for nesprin-1-α2 was most intense at the nuclear rim, but also faintly in the cytoplasm, in those fibres that were regenerating. b Serial sections from the same donor, stained for nesprin-1-giant (mAb N1G-Ex130; upper frame) and neonatal myosin (MHCn; lower frame) indicated that nesprin-1-giant was detected in all nuclei, but staining for nesprin-1-giant at the nuclear rim was more intense, with faint cytoplasmic staining, in regenerating fibres. White arrows indicate the nuclei of regenerating fibres in the paired serial section

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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