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Table 3 Nesprin-1 monoclonal antibodies used in this study

From: Specific localization of nesprin-1-α2, the short isoform of nesprin-1 with a KASH domain, in developing, fetal and regenerating muscle, using a new monoclonal antibody

mAb name Isotype Epitope Western blot IMF
   Unique sequence preceding Exon 131 of N1-Giant   
N1alpha2-1H2 IgG1 VVAEDLSALRMAEDGC (aa 2-17 of unique N1-alpha-2 sequence) - ++ (myotubes, immature and regenerating fibres)
   Exon 130   
N1G-Ex130 IgG1 SKASEIEYKLGKVNDRC +++ +++
   Exons 81-84   
N1G-7D9 IgG1 QDKLPGSSA (Exon 82) +++ +
N1G-8C8 IgG1 - ++ +
N1G-6F7 IgG1 - + +
N1G-4C11 IgG2b EMIDQLQDKLP (Exon 82) + (cross reaction) + (cross reaction)
   Exons 84-86   
N1G-7C8 IgG1 - ++ ++
N1G-5A6 IgG1 - ++ +
N1G-9G5 IgG1 LGLYTILPSELSL (Exon 84) +++ +
N1G-6C9 IgG1 LKIRDQIQDK (Exon 84-85) + (cross reaction) +
   Exons143-146   
MANNES1A IgG1 - ++ ++++
MANNES1E [25] IgG1 - +++ +++
  1. Except for MANNES1A and MANNES1E (Randles et al., 2010 [26]), all other Nesprin-1 mAbs in the table are reported here for the first time
  2. Exon numbering is based on the SYNE1 transcript variant 1 mRNA (accession: NM_182961.3) and is the same as that used by Rajgor et al., 2012 [27]