Specific localization of nesprin-1-α2, the short isoform of nesprin-1 with a KASH domain, in developing, fetal and regenerating muscle, using a new monoclonal antibody

Table 3 Nesprin-1 monoclonal antibodies used in this study

mAb name	Isotype	Epitope	Western blot	IMF
		Unique sequence preceding Exon 131 of N1-Giant
N1alpha2-1H2	IgG1	VVAEDLSALRMAEDGC (aa 2-17 of unique N1-alpha-2 sequence)	-	++ (myotubes, immature and regenerating fibres)
		Exon 130
N1G-Ex130	IgG1	SKASEIEYKLGKVNDRC	+++	+++
		Exons 81-84
N1G-7D9	IgG1	QDKLPGSSA (Exon 82)	+++	+
N1G-8C8	IgG1	-	++	+
N1G-6F7	IgG1	-	+	+
N1G-4C11	IgG2b	EMIDQLQDKLP (Exon 82)	+ (cross reaction)	+ (cross reaction)
		Exons 84-86
N1G-7C8	IgG1	-	++	++
N1G-5A6	IgG1	-	++	+
N1G-9G5	IgG1	LGLYTILPSELSL (Exon 84)	+++	+
N1G-6C9	IgG1	LKIRDQIQDK (Exon 84-85)	+ (cross reaction)	+
		Exons143-146
MANNES1A	IgG1	-	++	++++
MANNES1E [25]	IgG1	-	+++	+++

Except for MANNES1A and MANNES1E (Randles et al., 2010 [26]), all other Nesprin-1 mAbs in the table are reported here for the first time
Exon numbering is based on the SYNE1 transcript variant 1 mRNA (accession: NM_182961.3) and is the same as that used by Rajgor et al., 2012 [27]

ISSN: 2661-8850