|
mAb name
|
Isotype
|
Epitope
|
Western blot
|
IMF
|
|---|
| | |
Unique sequence preceding Exon 131 of N1-Giant
| | |
|
N1alpha2-1H2
|
IgG1
|
VVAEDLSALRMAEDGC (aa 2-17 of unique N1-alpha-2 sequence)
|
-
|
++ (myotubes, immature and regenerating fibres)
|
| | |
Exon 130
| | |
|
N1G-Ex130
|
IgG1
|
SKASEIEYKLGKVNDRC
|
+++
|
+++
|
| | |
Exons 81-84
| | |
|
N1G-7D9
|
IgG1
|
QDKLPGSSA (Exon 82)
|
+++
|
+
|
|
N1G-8C8
|
IgG1
|
-
|
++
|
+
|
|
N1G-6F7
|
IgG1
|
-
|
+
|
+
|
|
N1G-4C11
|
IgG2b
|
EMIDQLQDKLP (Exon 82)
|
+ (cross reaction)
|
+ (cross reaction)
|
| | |
Exons 84-86
| | |
|
N1G-7C8
|
IgG1
|
-
|
++
|
++
|
|
N1G-5A6
|
IgG1
|
-
|
++
|
+
|
|
N1G-9G5
|
IgG1
|
LGLYTILPSELSL (Exon 84)
|
+++
|
+
|
|
N1G-6C9
|
IgG1
|
LKIRDQIQDK (Exon 84-85)
|
+ (cross reaction)
|
+
|
| | |
Exons143-146
| | |
|
MANNES1A
|
IgG1
|
-
|
++
|
++++
|
|
MANNES1E [25]
|
IgG1
|
-
|
+++
|
+++
|
- Except for MANNES1A and MANNES1E (Randles et al., 2010 [26]), all other Nesprin-1 mAbs in the table are reported here for the first time
- Exon numbering is based on the SYNE1 transcript variant 1 mRNA (accession: NM_182961.3) and is the same as that used by Rajgor et al., 2012 [27]
