Table 4 Relative expression of mRNA of nesprin-1 isoforms and markers of differentiation in normal human skeletal muscle
Fetal Skeletal Muscle | Adult Skeletal Muscle | |||||||
|---|---|---|---|---|---|---|---|---|
Fetal 1 | Fetal 2 | Fetal 3 | Adult 1 (30y) | Adult 2 (18y) | Adult 3 (67y) | Adult 4 (85y) | Adult 5 | |
N1-Giant | 269.0 ± 127.7 (8) | 290.3 ± 36.6 (4) | 5.3 ± 3.3 (5) | 172.4 ± 27 (5) | 113.6 ± 16.5 (4) | 119.2 ± 30.4 (6) | 47.5 ± 9.3 (5) | 86.8 ± 25.9 (3) |
N1-β1 | 2.4 ± 1.9 (5) | 1 (2) | <1 (2) | 21.3 ± 2.9 (4) | <1 (2) | <1 (2) | 8.2 ± 3.0 (2) | 9.8 ± 2.6 (3) |
N1-β2 | 8.0 ± 1.9 (4) | 124.4 ± 43.2 (8) | <1 (3) | 4.9 ± 1.7 (4) | <1 (2) | 1 (2) | <1 (3) | 1 (2) |
N1-α1 | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) |
N1-α2 | 120.8 ± 33.9 (4) | 66.2 ± 18.1 (5) | 10.3 ± 2.3 (5) | 17.8 ± 5.8 (4) | 48.6 ± 12.7 (6) | 140.3 ± 25.4 (5) | 30.1 ± 5.3 (5) | 55.4 ± 18.2 (3) |
CKM | 4237 ± 95 (4) | 1611 ± 295 (4) | 3404 ± 971 (5) | 33117 ± 2320 (3) | 89214 ± 10651 (4) | 117928 ± 25917 (4) | 20372 ± 1290 (4) | 19033 ± 1851 (4) |
Desmin | 11928 ± 802 (3) | 3260 ± 1034 (6) | 14542 ± 3431 (5) | 38904 ± 1567 (3) | 84550 ± 20608 (4) | 82022 ± 23108 (5) | 37307 ± 617 (5) | 27197 ± 4613 (4) |