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Fig. 1 | BMC Cell Biology

Fig. 1

From: Extra-renal locations of the a4 subunit of H+ATPase

Fig. 1

Coronal section of posterior nasal cavity (NC) and vomeronasal organ (VNO) at e16.5 stained for β-gal activity and eosin. The dotted line indicates the approximate location of the future olfactory epithelium (a). Apical membrane localisation of the a4 subunit in the OE in p5 animals and its absence in the RE (b). Apical membrane localisation of the F subunit in the OE of p5 animals (c). High powered images of OE showing apical and basolateral staining (arrow heads) of the B1 and a4 subunits at p5 and the F subunit at p30 respectively (d–f). The B1 and F subunit is maintained in an apical membrane location in −/− animals (g, h). Scale bars: 20 μm. Western blot analysis of total protein lysates from the OE of −/− and +/+ adult animals (40 μg) and total mouse kidney lysate (MKT) (20 μg) against various subunits of the proton pump (i). Sizes (kDa) are marked on the left with arrows marking the bands of interest on the right

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