Fig. 2

Female reproductive tract. Western blot of uterus lysates for the a4 and D subunits (a). Panels show uterus from +/+ non-pregnant mice (lanes 1–3), +/− uterus from around +/+ embryos (lanes 4, 6), +/− uterus from around −/− embryos (lanes 5, 7) (40 μg) and total mouse kidney (MKT) (20 μg). Sections of VYS stained for a4 (green) and F (red) from +/+ embryos (b, c) and −/− embryos (d, e). Lysates from VYS of −/− and +/+ embryos (40 μg) and total mouse kidney (MKT) (20 μg) probed with antibodies against various subunits of the proton pump (f). β-gal (arrow heads) and H + E counterstained radial section of placenta (g) from an e16.5 +/− embryo showing the labyrinthine layer (L), spongiotrophoblast layer (S) or maternal decidual cells (M). High powered image of β-gal/H + E stained maternal tissue (h). Western blot of placenta lysates from +/+ embryos, −/− embryos (40 μg) and total mouse kidney (20 μg) blotted with α-a4 and α-C2 (i). All scale bars: 20 μm