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Fig. 1 | BMC Cell Biology

Fig. 1

From: Kinesin-2 and kinesin-9 have atypical functions during ciliogenesis in the male gametophyte of Marsilea vestita

Fig. 1

Characterization of the kinesin-2 and kinesin-9 families in Marsilea. ab Maximum likelihood trees were constructed using the motor domain from well-established kinesin-2 and -9 sequences. The motor domain from kinesin-1 (Kif5) was used as an out-group for these analyses. a Kinesin-2 motors can be separated into three subfamilies; kinesin-2α (red), -2β (orange), and -2γ (green). There are also many kinesin-2 sequences that do not correspond to any well-supported subfamily. The kinesin-2 motor in Marsilea (MvKinesin-2) is most similar to the kinesin-2 in Physcomitrella and does not fall into a kinesin-2 subfamily. b Kinesin-9 can be separated into two well-supported subfamilies, kinesin-9A (red) and kinesin-9B (blue). Marsilea has one kinesin-9A (MvKinesin-9A) and one kinesin-9B (MvKinesin-9B). c An increase in MvKinesin-2, -9A, and -9B mRNA abundance was detected using RT-PCR against poly(A+)-RNA isolated at 1–2, 3–5, and 6–8 h of gametophyte development. The abundance of centrin mRNA does not change during development and was used as a control

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