Fig. 2

Kinesin-2 and kinesin-9 are involved in spermatogenesis. a Unique 350–400 nt regions used for constructing dsRNA. b dsRNA constructed from MvKinesin-2, -9A, and -9B, respectively, using poly(A+)-RNA isolated at 8 h as a template. c RT-PCR for MvKinesin-2, MvKinesin-9A, MvKinesin-9B, and centrin respectively, in the absence (-) of any dsRNA at 8 h of development. d–f RT-PCR for MvKinesin-2, MvKinesin-9A, MvKinesin-9B, and centrin, respectively, in the presence (+) of (d) MvKinesin-2 dsRNA, (e) MvKinesin-9A dsRNA, or (f) MvKinesin-9B dsRNA. Each transcript cannot be detected after its knockdown. Centrin mRNA does not change after the addition of various kinesin dsRNAs. g Untreated, control microspores developed for 8 h, embedded in methacrylate, sectioned, and stained with TBO. Spermatogenous (SP) and jacket (j) cells can easily be distinguished from each other. Sectioned microspores treated with (h–i) MvKinesin-2, (j) MvKinesin-9A, and (k) MvKinesin-9B dsRNA and stained with TBO