Fig. 3

Spermatid differentiation is incomplete after silencing. a Untreated sectioned microspores developed for 8 h and labeled with anti-centrin Ab (red) and DAPI (blue). Centrin stains basal bodies (BB) and DAPI labels the nuclear coil. Sectioned 8 h microspores treated with (b–c) MvKinesin-2, (d) MvKinesin-9A, and (e) MvKinesin-9B dsRNA. b Centrin staining is diffuse and there are very few centrin aggregates that resemble basal bodies. The nuclear coil is not properly formed. c A small percentage of MvKinesin-2 knockdowns have a normal pattern of anti-centrin and nuclear staining. d Centrin staining is found throughout each spermatid and aggregates that resemble basal bodies are not localized at regular intervals adjacent to the nuclear coil. Most cells have a nuclear coil that appears similar to controls. e The blepharoplast (b), a centrosome like particle, is visible. Nuclei in these spermatids have failed to coil and differentiation has not occurred