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Fig. 5 | BMC Cell Biology

Fig. 5

From: Kinesin-2 and kinesin-9 have atypical functions during ciliogenesis in the male gametophyte of Marsilea vestita

Fig. 5

MvKinesin-9A knockdowns have irregularly positioned cilia. ad After spermatozoids emerged from the microspore, they were fixed in 2 % PFA on a coverslip and visualized with DIC microscopy. a Untreated cells have a characteristic corkscrew shape and cilia can been seen emerging from the microtubule ribbon and organelle coil. Developing microspores treated with (b) MvKinesin-9A, (c) MvKinesin-2, and (d) MvKinesin-9B dsRNA. b Cilia that appear to emerge from all parts of the spermatozoid cell body, not just the coil. c Spermatozoids are larger than controls and do not have the characteristic corkscrew shape. d Spermatozoids appear normal and look similar to controls

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