Fig. 6

MvKinesin-9A is required to position of basal bodies during gametogenesis. a–c Untreated, control microspores developed for 10.5 h, embedded in methacrylate, sectioned, and stained with (a) TBO or (b–c) anti-centrin antibody (red) and DAPI (blue). c Fluorescence images are overlaid on phase contrast images of the same microspore. The organelle coil as well as the ciliary band (CB) is detectable in most spermatogenous cells. Anti-centrin labeled basal bodies (BB) are most prominent anterior to the nuclear coil in the area containing the ciliary band. Microspores treated with (d–f) MvKinesin-2, (g–i) MvKinesin-9A, or (j–l) MvKinesin-9B dsRNA and allowed to develop for 10.5 h before being embedded in methacrylate, sectioned, and stained with (d, g, j) TBO or (e, f, h, i, k, l) anti-centrin antibody and DAPI. f, i, l Fluorescence images are overlaid on phase contrast images of the same microspore. d–f Spermatogenous cells are larger and divisions between the cells are unclear. Centrin staining is diffuse although some centrin aggregates that resemble basal bodies can be found. g–i The ciliary band is at irregular angles to the nuclear coil and is not clearly defined. Basal bodies are not restricted to regions anterior to the nuclear coil but are randomly localized throughout each spermatid. j–l The nuclear coil and ciliary band are not defined. Basal bodies are apparent and are localized at regular intervals along the nuclear coil, similar to at 8 h of development