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Table 1 Cytogenetic karyotyping analysis of clones derived of the JM8 parental line

From: Aneuploidy screening of embryonic stem cell clones by metaphase karyotyping and droplet digital polymerase chain reaction

  Number of clones (percentage of 708 clones analysed)
≥50 % euploidy 569 (80.5 %)
<50 % euploidya 138 (19.5 %)
ES clones with Chr 1 aneuploidyb 11 (1.6 %)
ES clones with Chr 8 aneuploidyb 119 (16.8 %)
ES clones with Chr 11 aneuploidyb 38 (5.4 %)
ES clones with Chr Y aneuploidyb 36 (5.1 %)
ES clones with at least four aneuploid chromosomes including 1, 8, 11 and Y 2 (0.3 %)
ES clones with three aneuploid chromosomes (at least two of these were among 1, 8, 11 and Y) 10 (1.4 %)
ES clones with two aneuploid chromosomes (at least one of these were among 1, 8, 11 and Y) 45 (6.4 %)
ES clones with one aneuploid chromosome among 1, 8, 11 and Y 81 (11.4 %)
ES clone with aneuploid chromosomes other than 1, 8, 11 or Y 1 (0.1 %)
ES clones with only aneuploid Chr 1 only 3 (0.4 %)
ES clones with only aneuploid Chr 8 only 64 (9.0 %)
ES clones with only aneuploid Chr 11 only 6 (0.8 %)
ES clones with only aneuploid Chr Y only 2 (0.3 %)
  1. The table shows the percentage and number of clones normal or with given aneuploidy (1, 8, 11, and Y) and summarises the cytogenetic karyotype analysis data generated at MRC Harwell
  2. aThose clones carrying multiple abnormalities are included in more than one category and as such the numbers recorded in the abnormality columns may exceed that recorded in the < 50 % euploidy category
  3. bOnly this aneuploidy or in combination with others aneuploidies