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Fig. 4 | BMC Cell Biology

Fig. 4

From: Prolactin-induced PAK1 tyrosyl phosphorylation promotes FAK dephosphorylation, breast cancer cell motility, invasion and metastasis

Fig. 4

Silencing of tyrosine phosphatase PTP-PEST rescues FAK auto-phosphorylation in T47D and TMX2-28 cells. a PTP-PEST siRNA reduces PTP-PEST mRNA in T47D and TMX2-28 cells. T47D and TMX2-28 cells were transfected with either PTP-PEST siRNA or control non-coding siRNA (ctrl) and mRNA levels were assessed by RT-PCR using PTP-PEST-specific primers. GAPDH primers were used an internal control. b WCL of T47D and TMX2-28 clones transfected with control or PTP-PEST siRNAs and treated with PRL (200 ng/ml) for 0 or 15 min were probed for FAK auto-phosphorylation using αpY397-FAK antibody. The expression levels of γtubulin were used as an internal loading control. c Graph represents the densitometric analysis of the bands obtained for pY397-FAK normalized to total FAK

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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