Fig. 3

Constitutively active derivatives of FMNL2 induce stress fiber formation. a Schematic of FH1-FH2 + C containing derivatives of each FMNL2 isoform. b FH1-FH2 + C containing derivatives of FMNL2 were expressed in NIH 3T3 cells by transient transfection. Expression of each of the FMNL2 isoforms (red) was sufficient to induce stress fiber formation (F-actin, green). Nuclei were detected with DAPI in the merged image. c NIH 3T3 fibroblasts were transiently transfected with an SRF luciferase reporter gene and the indicated FMNL2 derivative. Reporter activation is expressed relative to an SRF-VP16 control fusion protein. N = 3, error bars = SEM. Equivalent samples of transfected cell lysates were subjected to SDS-PAGE and immunoblotted using an anti-myc antibody (inset)