Fig. 4

The hif-1-dependent gene tyr-2 is overexpressed in wild type and djr-1.1 mutants but not in hif-1 animals treated with EtBr. The transcript level of tyr-2 was determined by qRT-PCR in wild type, hif-1 and djr-1.1 mutant worms grown on worm plates in the absence and presence of 50 μg/ml of EtBr. The experiment was performed independently at least three times. GraphPad Prism 5.0 was used to find statistical significance. Asterisk marks denote statistical significant differences (* denotes p < 0.05, ** p < 0.01, *** p < 0.001) in tyr-2 expression in wild type and djr-1.1 between untreated and EtBr treated samples but not in hif-1 animals