Fig. 2

Analysis of nuclear positioning of IGF2 transcripts relative to the proximal chromosome territory (SSC2) in interphase nuclei of porcine liver cells by 3D FISH and confocal microscopy. a, Two images of 3D RNA-DNA FISH revealing IGF2 RNA signals (green) and SSC2 (red): (I: IGF2 RNA at the edge SSC2 and II: IGF2 RNA outside SSC2). Nuclei were counterstained with DAPI; b, The distribution of IGF2 RNA towards the chromosome territory (SSC2) was scored in one of three classes: inside, edge and outside. The three classes were defined by combining the 3D distances (RNA spot center to chromosome territory edge and the percentage of colocalization (RNA in its chromosome territory) obtained with NEMO [29]. A significant fraction of RNA signals was found located outside SSC2 (in 72 % of nuclei)