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Fig. 3 | BMC Cell Biology

Fig. 3

From: Expressed alleles of imprinted IGF2, DLK1 and MEG3 colocalize in 3D-preserved nuclei of porcine fetal cells

Fig. 3

Position of expressed and non-expressed IGF2 alleles, relative to chromosome territories (SSC2). a, Extended focus of image sections from confocal microscopy created using Volocity software illustrating three scenarios (lines I, II, III) of IGF2 allele position towards SSC2: (I (out-out), II (edge-out) and III (in-in)). IGF2 gene transcription sites were detected by 3D RNA FISH, IGF2 DNA alleles and SSC2 by 3D DNA FISH. Column 1 shows the IGF2 RNA (green) and SSC2 (red), column 2 IGF2 DNA (green) and SSC2 (red), column 3 the merge. The nuclei were counterstained with DAPI (blue), the green fluorescence outside the nucleus is due to autofluorescence. White arrows on merge images indicate the position of the expressed alleles based on RNA FISH signals; b, Histogram representing the percentage of nuclei of the two groups of IGF2 alleles (expressed or not) relative to SSC2 (inside, edge or outside) in 51 nuclei

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