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Fig. 4 | BMC Cell Biology

Fig. 4

From: Expressed alleles of imprinted IGF2, DLK1 and MEG3 colocalize in 3D-preserved nuclei of porcine fetal cells

Fig. 4

Analysis of the association between IGF2 and (DLK1-MEG3) in liver cells. Decomposed confocal images (maximal intensity projections) were processed using Volocity software. The nucleus was counterstained with DAPI (blue). a, 3D DNA FISH: IGF2 – (DLK1/MEG3). I: IGF2 alleles (red), II: (DLK1-MEG3) alleles (green), each green spot corresponds to both merged alleles of DLK1 and MEG3 genes, III: merge, white arrows point to associated signals. On the right (IV); b, 3D RNA-DNA FISH: IGF2 RNA – (DLK1/MEG3) DNA. I: IGF2 RNA (green), II: (DLK1-MEG3) alleles (red), III: merge, white arrows point associated signals. On the left (IV): Histogram presenting the percentage of nuclei with RNA-DNA association was shown

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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