Skip to main content
Fig. 2 | BMC Cell Biology

Fig. 2

From: Intermittent hypoxia causes NOX2-dependent remodeling of atrial connexins

Fig. 2

The abundance and sizes of Cx40- and Cx43-containing gap junctions are reduced in atria of wild type C57BL/6 J mice exposed to intermittent hypoxia. Cx43 and Cx40 were localized by double label immunofluorescence in frozen sections of atria from mice treated with RA or IH. a Representative photomicrographs show localization of Cx43 (green), Cx40 (red) and their co-localization (white). Bar, 20 μm. Both Cx43 and Cx40 are detected in RA and IH samples; levels of each appear decreased in the IH images; but, the extent of their overlap did not differ between RA and IH. (See text for quantitation of overlap.) b Graph depicts the quantitation of the abundance of Cx43- and Cx40-containing gap junctions in these samples (based on image analysis as described in Material and Methods). The abundances of both Cx43 and Cx40 immunofluorescence per cell area were significantly reduced in atria from IH mice (*, p < 0.05, Student’s t test, n = 18 for RA, black bars; n = 16 for IH, grey bars). c Graph depicts the quantitation of the sizes of Cx43- and Cx40-containing gap junctions in these samples. The sizes of Cx43 or Cx40 immunoreactive particles were significantly reduced in atria from IH mice (*, p < 0.05, Student’s t test, n = 18 for RA, black bars; n = 16 for IH, grey bars)

Back to article page