Fig. 4
From: Hierarchical imaging: a new concept for targeted imaging of large volumes from cells to tissues
Organelle inventories of different cells in a zebrafish immune cell population isolated from spleen. Overview of ROI imaged with 5 nm pixel size (a), containing RBCs (red, b, c), cytotoxic cells (green, d, g), neutrophils (cyan, e, h) and basophils (yellow, f, i). Representative cross sections from 3D data sets of 40–100 sections of these cells (b, d-f), a volume rendering for an RBC (c) and segmented 3D data (g, h, i) showing nucleus in dark blue, ER in light blue, mitochondria in red, Golgi apparatus and secretory lysosomes in yellow, neutrophilic granules in brown, basophilic granules in gold, and plasmamembrane in green. Scale bars, 2 μm