Fig. 2
From: miR-27b inhibits fibroblast activation via targeting TGFβ signaling pathway
Effect of miR-27b on fibroblast activation. LL29 fibroblasts were infected with a miR-27b lentivirus or virus control (VC) at a MOI of 50. a miR-27b expression and b mRNA expression of COL1A1, COL3A1, COL4A1, and α-SMA were determined by real-time PCR. The expression levels of miR-27b and mRNA were normalized to U6 and GAPDH, respectively. mRNA levels were expressed as fold changes over the control group without TGFβ1 treatment (CON). c, d The protein level expression of α-SMA and COL3A1 were determined by western blotting and quantitated using Image J software. The results were normalized to β-actin and expressed as fold changes over control without TGFβ1 treatment (CON). Data shown are means ± S.E, *P < 0.05, **P < 0.01. n = 3 for a and d, n = 6–10 for b. Student t-test for a and ANOVA, followed by Turkey’s test for b and d