Fig. 3From: Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expressionReduced primitive endoderm differentiation of ES cells deficient of lamin A/C and/or emerin. a A Western blot shows the absence of lamin A/C and/or emerin proteins in ES cell lines with lmna (−/−) and/or emd (−/−) genotypes. Cells were incubated for 4 days with 1 μM RA, and cell lysates were prepared. b Wild type, lmna (−/−) and/or emd (−/−) ES cells were treated with or without RA for 4 days. The cell lysates were analyzed by Western blot. Protein loading was normalized to total (pan) ERK1/2. c The undifferentiated and differentiated cells were analyzed by qRT-PCR for expression of various markers. Values for each sample were normalized to respective GAPDH levels and plotted relative to control, untreated wild type (wt), which is defined as “1”Back to article page