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Table 1 Nuclear measurements in blastocysts. Confocal imaging analysis was used to determine the x-, y-, and z-dimensions of nuclei in E4.5 blastocysts. The volumes of the nuclei were calculated from the measurements, as described in Methods

From: Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression

Blastocyst Nuclei

Oct4 + / Gata6 – ICM cells

Oct4 - / Gata6 + Trophectoderm

Oct4 +/− / Gata6 +Primitive endoderm

(μm)

Mean ± S.D.

Mean ± S.D.

Mean ± S.D.

x- dimension

13.3 ± 0.27

10.98 ± 0.41

11.99 ± 0.56

y- dimension

16.8 ± 2.36

13.5 ± 0.43

12.5 ± 2.25

z- dimension

8.73 ± 1.12

8.78 ± 0.55

7.79 ± 1.51

Volume (μm3)

1001 ± 129

688 ± 73

616 ± 126