Fig. 2

Nuclear division occurs within the appressorium. a M. oryzae CKF1962 (derived from O-137) infecting rice cultivar YT16. Shown are single plane confocal images of both red fluorescence and bright-field (top), and red fluorescence alone (shown in white; bottom). Nuclei are denoted by arrowheads. A single nucleus tagged with tdTomato (red) separated into two mitotic nuclei within the appressorium. One nucleus then migrated out of focus into the primary hypha, while the other remained in the appressorium. b M. oryzae Guy11-derived transformant infecting YT16 at the same growth stage as (a). Nuclear dynamics were consistent with (a), with nuclear division occurring within the appressorium (double arrowheads) and migration of a single nucleus out of focus into the primary hypha. c M. oryzae Guy11-derived transformant infecting rice cultivar CO-39 at the same growth stage as both (a) and (b). Nuclear dynamics were again consistent with nuclear division occurring in the appressorium (double arrowheads) and migration of one nucleus into the primary hypha. Bar = 5 μm