Fig. 3

The first post-penetration septum forms in the primary hypha. Shown are maximum intensity projections of confocal optical sections of a rice cell invaded by M. oryzae strain CKF2686 at 27 hpi. Merged nuclear tdTomato (red) and GFP fused to PLCdelta:PH (green) are shown. Top: One nucleus (arrowhead) was located in the appressorium (outlined by dotted line) and GFP-PLCdelta:PH was localized to the plasma membrane. Middle: The same infection site imaged 50 min later showing one appressorial nucleus and one nucleus in the bulbous hypha. Bottom: The same infection site after an additional 25 min showing GFP fluorescence concentrating at the location of the first septum (arrow). Bar = 5 μm