Fig. 5

Hyphal morphology in heat-killed rice cells is distinct from growth in live cells. M. oryzae CKF2138, expressing cytoplasmic EYFP (green) and nuclear tdTomato (red, but shown in yellow due to overlap of green and red), growth in heat-killed rice cells at 25 hpi. Shown are merged fluorescence confocal images of a single focal plane (top and middle), or a projection (29 μm in total depth) (bottom) of the same infection site. Rice cell walls are shown as white lines. Top: A single focal plane shows the appressorium (asterisk) with a nucleus, germ tube (star), and hypha (x) that grew on the rice surface. Middle: A focal plane 12 μm beneath the appressorium shows 2 filamentous-like IH branches (plus signs) and locations where IH had crossed the rice cell wall into neighboring cells (arrows). Bottom: A maximum intensity projection shows all features of both focal planes (top and middle). Bar = 10 μm