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Fig. 1 | BMC Cell Biology

Fig. 1

From: Variation in human dental pulp stem cell ageing profiles reflect contrasting proliferative and regenerative capabilities

Fig. 1

Characterisation of DPSC populations derived from human dental pulp tissues. a Cumulative population doublings (PDs) were recorded for six DPSC populations during extended culture until senescence was reached, when PDs reached <0.5 PDs / week. DPSCs A1, A2 and A3 were derived from patient A, while B1, C2 and C3 were derived from patients B and C, respectively. Population A3 demonstrated high proliferative capacity, achieving >80 PDs over 280 days in culture, whilst the other DPSC populations only achieved 20–35 PDs over 35–85 days in culture. b Telomere length analysis for the six DPSC populations at cited PDs, by Southern blotting. Average telomere lengths were calculated using ImageJ® Software. Much longer telomeres were identified with high proliferative capacity, A3 (18.9 kb), compared with low proliferative capacity populations, A1 (5.9 kb), A2 (9.6 kb), B1 (5.6 kb), C2 (7.2 kb) and C3 (12.8 kb). A3 (18.9 kb, 9 PDs) only exhibited equivalent telomere lengths to low proliferative capacity DPSCs, at much later PDs towards the end of its proliferative lifespan (6.4 kb, 55 PDs). CTRL lanes represent the separate DIG-labelled, Control DNA sample. Average telomere lengths values obtained for each CTRL lane from left-right, were 10.6 ± 0.73 kb, 11.4 ± 0.71 kb and 11.1 ± 0.60 kb, respectively. Separated DIG-labelled telomere length standards are also included. c Correlation between cumulative PDs versus original telomere lengths for all DPSCs analysed

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