Fig. 2
Gene expression analysis of DPSC populations by RT-PCR, at early PDs. All DPSCs showed positive expression for the MSC markers, CD73, CD90 and CD105; and the absence of the hematopoietic marker, CD45. All DPSCs analysed were also negative for hTERT. Only low proliferative capacity DPSCs (A1, A2, B1, C2 and C3) were positive for the expression of CD271 (nerve growth factor receptor p75, LNGFR) and for all 3 senescence-associated marker genes analysed, p53, p21waf1 and p16INK4a. Replacement of cDNA with H2O served as negative controls, with β-actin serving as the reference housekeeping gene