Table 1 Details of the primer sequences and cycling conditions used for RT-PCR
Gene marker | Primer sequence | Annealing temp. (°C) | Cycles | NCBI reference sequence |
|---|---|---|---|---|
CD73 | F:5′GTCGCGAACTTGCGCCTGGCCGCCAAG-3′ R: 5′-TGCAGCGGCTGGCGTTGACGCACTTGC-3′ | 65 | 35 | NM_001204813.1 |
CD90 | F:5′- ATGAACCTGGCCATCAGCATCG-3′ R:5′- CACGAGGTGTTCTGAGCCAGCA-3′ | 55 | 35 | NM_006288.3 |
CD105 | F:5′-GAAACAGTCCATTGTGACCTTCAG-3′ R: 5′-GATGGCAGCTCTGTGGTGTTGACC-3′ | 65 | 35 | NM_001114753.2 |
CD271 | F:5′- CTGCAAGCAGAACAAGCAAG-3′ R:5′- GGCCTCATGGGTAAAGGAGT-3′ | 55 | 35 | NM_002507.3 |
CD45 | F:5′-GTGACCCCTTACCTACTCACACCACTG-3′ R:5′-TAAGGTAGGCATCTGAGGTGTTCGCTG-3′ | 65 | 35 | NM_002838.4 |
hTERT | F:5′- CGGAAGAGTGTCTGGAGCAA-3′ R:5′- GGATGAAGCGGAGTCTGGA-3′ | 55 | 40 | NM_198253.2 |
p53 | F:5′- AGACCGGCGCACAGAGGAAG-3′ R:5′- CTTTTTGGACTTCAGGTGGC-3′ | 55 | 35 | NM_001126118.1 |
p21waf1 | F:5′- GGATGTCCGTCAGAACCCAT-3′ R:5′- CCCTCCAGTGGTGTCTCGGTG-3′ | 60 | 35 | NM_001291549.1 |
p16INK4A | F:5′- CTTCCTGGACACGCTGGT-3′ R:5′- GCATGGTTACTGCCTCTGGT-3′ | 55 | 35 | NM_001195132.1 |
OCN | F:5′-GCAGGTGCGAAGCCCAGCGGTGCAGAG-3′ R: 5′-GGGCTGGGAGGTCAGGGCAAGGGCAAG-3′ | 62 | 35 | NM_199173.4 |
OPN | F:5′- ATCACCTGTGCCATACCA-3′ R:5′- CATCTTCATCATCCATATCATCCA-3′ | 55 | 35 | NM_001251830.1 |
PPARγ | F:5′-GCCATCAGGTTTGGGCGGATGCCACAG-3′ R: 5′-CCTGCACAGCCTCCACGGAGCGAAACT-3′ | 62 | 35 | NM_138711.3 |
LPL | F:5′-GCTGGCATTGCAGGAAGTCTGACCAATAA-3′ R:5′-GGCCACGGTGCCATACAGAGAAATCTCAA-3′ | 55 | 35 | NM_000237.2 |
β-actin | F:5′-AGGGCAGTGATCTCCTTCTGCATCCT-3′ R:5′- CCACACTGTGCCCATCTACGAGGGGT-3′ | 65 | 35 | NM_001101.3 |