Fig. 6

BMP-12 treatment impairs immunomodulatory properties of hASCs. The effect of hASCs treated or not with BMP-12 (a. 50 ng/ml and b. 100 ng/ml) for 7 days on proliferation of PBMCs (peripheral blood mononuclear cells) in Mixed Lymphocytes Reaction (MLR). PBMCs from two independent donors X and Y were used for each experiment. Isolated PBMCs were stimulated in autologous or allogeneic manner (by addition of irradiated PBMCs from the same or second donor respectively). Mixture was cultured with or without addition of hASCs (treated with BMP-12 or not). +/- indicates the presence of a given component. Data presented as means and SEM. Results analyzed with Student T test or non-parametric Wilcoxon test depending on distribution. Letters above bars indicate statistical differences: groups sharing the same letter do not differ significantly (p < 0.05). Number of hASCs donors n = 5, each hASC population tested with PBMCs from two different donors (X and Y). The amount of 3H-thymidine incorporated into the cells giving the level of radioactivity as ccpm – corrected counts per minute, tests performed in triplicates