Table 1 Primers for mRNA expression
From: Hinokitiol induces DNA demethylation via DNMT1 and UHRF1 inhibition in colon cancer cells
Genes | Primer sequences (5′ → 3′) | Annealing temp. (°C) | Product size (bp) |
|---|---|---|---|
DNMT1 | F:AGACTACGCGAGATTCGAGTC | 60 | 171 |
R:TTGGTGGCTGAGTAGTAGAGG | |||
MGMT | F:ACCGTTTGCGACTTGGTACT’ | 54 | 268 |
R:CGGGGAACTCTTCGATAGCC | |||
AKR1B1 | F:CCCATGTGTACCAGAATGAGAA’ | 58 | 362 |
R:CTGGAGATGGTTGAAGTTGGAG’ | |||
CHST10 | F:GTGTGATTGGACACCACGAG’ | 58 | 176 |
R:ATACAGGCGTCGGATGTCTC’ | |||
BTG4 | F:GCAAGGAACCTCGTGTCATT | 58 | 159 |
R:GCGAGCCATGGTAAGTGTTT’ | |||
SPG20 | F:CCAACTGGAACAGAGCAGAAG’ | 58 | 160 |
R:TTAGCCCTTTTTCCACGTTTT’ | |||
β-actin | F:AGAGCTACGAGCTGCCTGAC | 60 | 184 |
R:AGCACTGTGTTGGCGTACAG |