Table 2 Primers for quantitative methylation-specific PCR (QMSP)
From: Hinokitiol induces DNA demethylation via DNMT1 and UHRF1 inhibition in colon cancer cells
Gene | Primer sequences (5′ → 3′) | Annealing temp. (°C) | Product size (bp) | Location | Gene bank |
|---|---|---|---|---|---|
NEUROG1 | F:TATGTAAATATTCGGGCGTTGTAC | 58 | 199 | (−155 ~ +43) | NC_000005.9 |
R:GATCTCCTAAATAATATCGCCGAC | |||||
TAC1 | F:TTAGATTTGTAGACGGAAGTAGGTC | 58 | 139 | (−135 ~ +3) | NC_000007.13 |
R:GTAATTAAAAATTTCCGAAACGAT | |||||
MGMT | F:GTTTGTATTGGTTGAAGGGTTATTT | 58 | 109 | (−292 ~ −184) | NC_000010.10 |
R:CTAAAACAATCTACACATCCTCACT | |||||
AKR1B1 | F:CGGAAGAAGTATTTTCGTCGA | 58 | 166 | (−120 ~ +45) | NC_000007.13 |
R:CAATACGATACGACCTTAACCG | |||||
CHST10 | F:TTTTGTAGCGGTAGAAAGGGAGATTCG | 58 | 198 | (−125 ~ +72) | NC_000002.11 |
R:GACTTTAAAAACCAAAACGCCGAC | |||||
BTG4 | F:GTATAATACGCGTAGTTGGGTTAGC | 58 | 171 | (−422 ~ −252) | NC_0000011.9 |
R:AAAAAAACGAAAAAAACCTAAACG | |||||
ELOVL4 | F:GAGTTTAGGTGTTTCGTTTTCGTTC | 58 | 114 | (−158 ~ −42) | NC_000006.11 |
R:CCTCCCTCCCTAATATTAAAACTCG | |||||
EYA4 | F:GTTATTCGAGGTTAAATAAAAACGG | 58 | 149 | (−198 ~ −50) | NC_000006.11 |
R: ACTTACGCAAAAAAATAAAACGAA | |||||
SPG20 | F:GTCGAGTAGTCGACGTGGTC | 58 | 168 | (−246 ~ −78) | NC_000013.10 |
R:AATAATACGTAAAAAAACGTCCGTC | |||||
UNC5C | F:GTTTAGGTTTGGCGTATCGC | 58 | 219 | (−463 ~ −245) | NC_000004.11 |
R:GCCAAAAAAACGTAAAAAACG | |||||
β-actin | F:TGGTGATGGAGGAGGTTTAGTAAGT | 58 | 132 | (−1645 ~ −1513) | NC_000007.13 |
R: AACCAATAAAACCTACTCCTCCCTTAA |