Fig. 6

Flow cytometry analysis of MEF2 and ACTN2 in MSC and myoblast co-cultures, MSC and myoblast monocultures and L6-myoblasts. Markers are presented with mean +/- SD. a Highly significant upregulation of MEF2 in co-cultures from 2 to 14 d of stimulation with HGF + IGF-1. Higher levels of MEF2 in MSC monocultures could be observed in stimulated and control groups compared with L6-myoblasts. The expression of MEF2 was slightly downregulated after 14 d in myoblast (Mb) monocultures. b Highly significant upregulation of ACTN2 in co-cultures both under HGF + IGF-1 and in control groups after 14 d compared with 2 d. After 2 d of cultivation, the lowest levels of ACTN2 were demonstrated in MSC monocultures. A 2.7-fold upregulation in unstimulated controls and 3.2-fold under HGF + IGF-1 was observed in MSC monocultures after 14 d. The expression of ACTN2 was downregulated in stimulated and control Mb monocultures. Higher expression of ACTN2 was observed when Mb were cultivated in control groups. (** = p ≤ 0.01). Mb of three different isolations as well as three replicates of each were used. One replicate of MSC and L6 was used