Skip to main content
Fig. 7 | BMC Cell Biology

Fig. 7

From: Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

Fig. 7

Intermittent PTH attenuated cementogenesis-related gene expression inhibition that was induced by mechanical strain. a Western blot analysis of BSP, ALP, OCN, COL1, Runx2 and Osx levels in cells of the control group, the strain group and the strain + PTH group that was treated with mechanical strain and intermittent PTH (also shown in Additional file 4: Figure S3). Except the control group, other two groups were both treated with 18 h mechanical strain, while the control group was placed in the bending box without mechanical strain. Then, the control group and the strain group were exposed to 3 cycles of vehicle treatment. Each cycle comprises 6 h vehicle medium and 18 h fresh medium. Meanwhile, the strain + PTH group were exposed to 3 cycles of intermittent PTH administration. Each cycle comprises 6 h PTH (50 ng/ml) medium and 18 h fresh medium. Results were representative of 3 experiments. b-g Densitometry of bands was performed and normalized to GAPDH. The control group was set to 1. Bars represent means ± SD of triplicate measurements. * p < 0.05

Back to article page