Table 1 Sequences of primers used in qPCR
Gene | Forward primer sequence (5’–3’) | Reverse primer sequence (5’–3’) |
|---|---|---|
GAPDH | GACATCAAGAAGGTGGTGAAGC | GAAGGTGGAAGAGTGGGAGTT |
ALP | CCAACTCTTTTGTGCCAGAGA | GGCTACATTGGTGTTGAGCTTTT |
BSP | TAGGAGTTTCCAGGTTTCTGATGA | CTGCCCTTTCCGTTGTTGTC |
COL1 | CTGGCGGTTCAGGTCCAAT | TTCCAGGCAATCCACGAGC |
OCN | TGCTTGTGACGAGCTATCAG | GAGGACAGGGAGGATCAAGT |
OPN | TAGGAGTTTCCAGGTTTCTGATGA | CTGCCCTTTCCGTTGTTGTC |
Osterix | CTCACCAGGTCCAGGCAACA | GGAGCAAAGTCAGATGGGTAAGTAG |
Runx2 | GGACGAGGCAAGAGTTTCACC | GAGGCGATCAGAGAACAAACTAGG |