Skip to main content
Fig. 2 | BMC Cell Biology

Fig. 2

From: Loss of pericyte smoothened activity in mice with genetic deficiency of leptin

Fig. 2

Leptin Deficiency Changes HSC Phenotype. Primary HSCs isolated from WT and ob/ob mice were cultured on plastic dishes in serum-containing medium for 2 h (d0) or 7 days (d7). a qRT-PCR and (b) FACS analysis of MF genes (αSMA, Col1α1, and PDGF), and (c) qRT-PCR analysis of quiescence genes (PPARγ, CD36, and GFAP) in quiescent and myofibroblastic HSCs. Results were normalized to WT d0 cells. Ɨ p < 0.05 vs WT, * p < 0.05 vs d0. n = 3. Red lines indicate Isotype controls. d Lipid content of cultured HSC at indicated days was examined by Oil Red O staining. (original magnification, ×10) (e) Migration and proliferation of HSCs were measured by scratch assay and BrdU assay. Results were normalized to WT d4 cells. Ɨ p < 0.05 vs WT, * p < 0.05 vs d0. n = 3. f Senescence was examined by β-galactosidase staining (blue) of HSCs cultured for 1, 4 and 7 days. (original magnification, ×10)

Back to article page