Fig. 5

Leptin Treatment Does Not Rescue Leptin-deficient HSCs (a) Primary HSCs isolated from either WT or ob/ob mice were cultured in serum-containing medium and treated with leptin (100 ng/ml) or vehicle for 3 days. RNA was isolated, and changes in gene expression of MF markers were evaluated by qRT-PCR. Results were normalized to WT HSCs treated with vehicle. Ɨ p < 0.05 vs WT, * p < 0.05 vs vehicle. b Leptin or vehicle was administrated subcutaneously (100 g/kg body weight per day) to ob/ob mice for 6 days. Primary HSCs were isolated and cultured for 7 days. qRT-PCR analysis of gene expression in freshly isolated and 7-day cultured HSCs. Results were normalized to d0 ob/ob HSCs treated with vehicle. Ɨ p < 0.05 vs WT, * p < 0.05 vs vehicle