Fig. 7From: Loss of pericyte smoothened activity in mice with genetic deficiency of leptinLeptin-deficient Adipose-derived Mesenchymal Stem Cells are Smoothened Resistant. Adipose stromal vascular fractions isolated from WT and ob/ob mice were plated and cultured for 3 weeks to derive homogeneous mesenchymal stem cell populations for RNA isolation. Hh-related genes and markers of adipocyte differentiation were evaluated by qRT-PCR. Results were normalized to WT cells. * p < 0.05 vs WTBack to article page