Fig. 2

SING formation correlates with reduced protein quality control. a Worms expressing GFP::Ub and RPT-1::mCh were incubated for 60 min at either 25 °C or 34 °C prior to soaking in 500 mM NaCl for 60 min. The merged image is shown. Areas that appear yellow have both GFP and mCherry. The brief heat shock reduced the occurrence of SING. Cytoplasmic granules also appear as a result of the heat shock. When hsf-1 was knocked down via RNAi, SING formation was normal even after heat shock. Scale bar indicates 10 μm. b The percentage of oocytes forming SINGs as described in A. A total of 1020 oocytes were observed for each condition from 3 independent experiments (n = 30 worms). Statistically relevant differences ***p < 0.0002, two-tailed z test. c Percentage of oocytes forming SINGs in day 1 and day 4 adults after exposure to 500 mM NaCl for the times indicated. A total of 510 oocytes were observed for each time point from 3 independent experiments (n = 30 worms). Statistical significance was calculated by a Fisher’s Exact test: **p < 0.01 and ****p < 0.0001