Fig. 7

SINGs form in intestinal cells. a A strain expressing GFP::Ub in intestinal cells was soaked in 1 M NaCl for 1 h prior to imaging with a laser scanning confocal microscope. The salt stress induced SING formation in intestinal nuclei. Worms that had been grown under ubc-18 (RNAi) did not form SINGs upon salt stress. b Quantification of the intestinal nuclear response to salt stress. Three trials were done for each condition including 10 worms per trial and 10 intestinal nuclei per worm (n = 30 worms). Statistical significance was calculated by a Fisher’s Exact test: ***p < 0.001