Fig. 8

SING formation does not require intertissue signaling. a Young adult worms expressing RPT-1::mCh were cut open in either M9 buffer or 500 mM NaCl to extrude the gonads from the body. Carcasses containing the other tissues were quickly removed. Gonads were imaged after 60 min. All gonads soaked in 500 mM NaCl formed SINGs (134/300 nuclei; 10 gonads). No SINGs were formed in the gonads soaked in M9 (0/300 nuclei; 10 gonads). Data were collected from 3 independent experiments (n = 10 worms). b Intestinal cells also form SINGs when separated from the body. Intestinal nuclei are large and polyploid and have an elongated shape. Worms expressing GFP::Ub in the intestine were cut open in either M9 buffer or 1 M NaCl to extrude the intestine from the body. Carcasses containing the other tissues were quickly removed. Intestines were imaged after 60 min. No intestines soaked in M9 formed SINGs (0/40 nuclei; 8 intestines). For intestines soaked in 1 M NaCl, all intestines showed SINGs (14/30 nuclei; 8 intestines). Data were collected from 2 independent experiments (n = 8 worms). Scale bar indicates 10 μm