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Fig. 2 | BMC Cell Biology

Fig. 2

From: Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation

Fig. 2

Stages of DPPSC osteogenic differentiation during 21 days. a Relative expression of pluripotency (OCT3/4, NANOG) markers during the osteogenic differentiation of DPPSC versus DPPSC at Day 0 (dotted line). b Relative expression of osteogenic markers (RUNX2, OC) during the osteogenic differentiation of DPPSC versus SAOS-2 cells differentiated over 21 days in osteogenic medium (dotted line). c RT-PCR for osteogenic markers (ALP, OC, COL1, RUNX2) at days 0, 11 and 21 of differentiation. Bone cDNA was used as a positive control and GAPDH was used as a housekeeping. d Proposed scheme for osteogenic DPPSC culture with recognizable stages of differentiation. *P < 0.05

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