Fig. 3

Osteogenic capacity of DPPSC during 21 days. a Cell morphology of DPPSC observed with optic microscopy at days 0, 3, 7, 15 and 21 of osteogenic differentiation. b ALP activity observed with optic microscopy at days 0, 3, 7, 15 and 21 by an ALP staining (blue). c Images of mineralitzation at days 0, 3, 7, 15 and 21 stained by the von Kossa method showing mineralized bone (brown) and osteoid, supporting tissue and structures (red). d, e Osteogenic protein expression of DPPSC after 21 days. d Immunofluorescence analysis of DPPSC showing the expression of Osteogenic markers at day 21 of differentiation. Hoechst (HT) as a nucleus control. Scale bars: 200 μm (a-c), 50 μm (d). e Western Blot analysis of osteogenic markers at day 0 and 21 of differentiation. SAOS-2 cells were used as a positive control. GAPDH was used as a housekeeping control